<p>Schema of the E-selectin gene and the location of the primers used in the ChIP assay. Chromatin was prepared from sparse and confluent HUVECs monolayers treated with (+) or without (−) 1 ng/ml TNFα for 30 min, and the ChIP assay was performed using the anti-acetyl histone H3 (AcH3) antibody. Real-time PCR analysis of the ChIP samples was performed using primers designed to amplify the E-selectin promoter region (−195 to −67), which contains the cytokine response region (CRR) and the E-selectin extreme 3′ region (+13914 to +13977) (*<i>p</i><0.01). The values are expressed as mean ± SD of three independent experiments.</p
