Efficient binding of the amino-terminal portion of GIT1 to the carboxy-terminal portion requires the inclusion of the first Spa2 region of the SHD.

Abstract

<p>(<b>A</b>) Scheme of the constructs of avian GIT1 used in the following experiment. To be noted that tyrosine 284 of avian GIT1 corresponds to tyrosine 293 of human GIT1. (<b>B</b>) COS7 cells were cotransfected with the indicated combination of HA-tagged amino-terminal and FLAG-tagged carboxy-terminal fragments of GIT1. Lysates were immunoprecipitated with the anti-FLAG M2-conjugated beads. Filters with immunoprecipitates (IP, from 250 μg of protein lysate) and lysates (50 μg) were incubated with anti-HA or anti-FLAG antibodies. Amino-terminal fragments in the lysates are indicated by asterisks.</p