<p>TEM images from three capes in wild-type nuclei. <b>A, B</b> – These images shows two capes where regions of perinuclear vesicles (PNV) and the terminal chamber (TC) are visible. NE – nuclear envelope. NP – nucleoplasm. As illustrated in A the PNV region is often associated with an out folding of the outer nuclear membrane (arrowhead), non-membrane bound granules (asterisk) and small vesicles (see D). Scale bars are 1 μm. <b>C</b> – Higher magnification view of part of the membrane from a terminal chamber in B (arrow) showing two membrane bilayers (arrowheads). Scale bar is 200 nm. <b>D</b>–A terminal chamber containing several vesicular and granular organelles (dashed line, asterisk). Two PNV are also visible at the periphery of this chamber (arrows). <b>E</b>–A second cape showing only the region of PNV. Scale bar is 2 μm. <b>F</b> – Higher magnification view of several PNV in C (dashed line with arrow). PNV are bounded by a single bilayer and are separated from the NP by a single bilayer and often protrude into one another. See also serial sections in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093680#pone.0093680.s003" target="_blank">Movie S1</a>. <b>G</b> – Chart showing the number of capes per nucleus with a given size (arbitrarily estimated in the Z dimension in serial block face SEM image series as the number of 50 nm sections from first appearance to disappearance in serial sections and normalized to the nuclear diameter in the same direction; see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093680#pone.0093680.s004" target="_blank">Movie S2</a>). Data are shown for wild-type (black bars) and AnxB9<sup>RNAi</sup> (grey bars). Many more small capes are present when AnxB9 is knocked down. <b>H</b> - Chart showing the number of fully sectioned nuclei containing capes with a terminal chamber. Data are shown for wild-type (black bars) and AnxB9<sup>RNAi</sup> (grey bars). Whereas all capes end in a terminal chamber in wild-type, few do when AnxB9 is knocked down.</p
