<p>(<b>A</b>) Scheme of donor vector pMos{rps9::egfp}<sup>frkt1074</sup>; in analogy to pTol2{rps9::egfp}<sup>frkt868</sup> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093076#pone-0093076-g001" target="_blank">Figure 1A</a>), the rps::egfp cassette is flanked by inverted Mos repeats (MosIR) that serve as recognition site for Mos1 transposase. (<b>B, C</b>) PCR using vector-specific primers (black arrows) yields a variety PCR fragment from embryos co-injected with <i>mos1</i> transposase mRNA (left lanes), but not controls (right lanes). (<b>D</b>) Sequencing of 21 individual PCR product reveals imprecise breakpoints caused by impaired excision of reporter constructs and/or subsequent modification of DNA ends in the context of non-homologous end repair. See <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093076#pone.0093076.s003" target="_blank">Alignments S1</a></b> and <b><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0093076#pone.0093076.s004" target="_blank">S2</a></b> for details.</p
