<p>(<b>A</b>) <i>In vivo </i><sup>35</sup>S labeling of CAV20-wt and CAV20-BSOr viral proteins in the absence and presence of BSO in HeLa H1 cells. Viral proteins were separated on SDS-polyacrylamide gels (12.5%), as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004052#s4" target="_blank">Materials and Methods</a>. (<b>B</b>) The genome structure of the CAV20 FLuc replicon used in the experiment is shown above. The FLuc coding sequence was used to replace the P1 domain of the polyprotein. RNA transcripts of the replicon were transfected into untreated or BSO-treated HeLa H1 cells either in the absence or presence of GnHCl (2 mM). Luciferase activity was measured at 9 hr post transfection. (<b>C</b>) CAV20 FLuc replicon RNA transcript was transfected into BSO-treated HeLa H1 cells either in the absence or presence of GnHCl. At 1 hr post-transfection the cells were superinfected either with wt or with CAV20-BSOr (VP3 Y97H) at a moi of 0.5. The cells were lysed at 6 hr post-infection and were then used to re-infect fresh HeLa H1 cells in the absence or presence of GnHCl.</p
