Aceriphyllum rossii Extract and Its Active Compounds,
Quercetin and Kaempferol Inhibit IgE-mediated Mast Cell Activation
and Passive Cutaneous Anaphylaxis
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Abstract
Aceriphyllum rossii contains an abundant source of
natural flavonoids with potential antioxidant, anticancer and anti-inflammatory
properties. However, the effect of A. rossii extract (ARE) on immunoglobulin E(IgE)-mediated allergic responses
remains unknown. In the present study, the effects of ARE and its
active compounds, quercetin and kaempferol, on IgE-mediated rat basophilic
leukemia mast cell activation and passive cutaneous anaphylaxis (PCA)
were investigated. ARE, quercetin, and kaempferol inhibited secretion
of β-hexosaminidase and histamine, and reduced the production
and mRNA expression of interleukin-4 and tumor necrosis factor-α.
ARE also decreased the production of prostaglandin E<sub>2</sub> and
leukotriene B<sub>4</sub> and expression of cyclooxygenase 2 and 5-lipoxygenase.
Furthermore, ARE, quercetin, and kaempferol inhibited IgE-mediated
phosphorylation of Syk, phospholipase Cγ, protein kinase C (PKC)μ,
and the mitogen-activated protein kinases, extracellular signal-regulated
kinase, p38, and c-Jun N-terminal kinase. ARE, quercetin, and kaempferol
markedly suppressed mast cell-dependent PCA in IgE-sensitized mice.
These results indicate that ARE and its active constituents, quercetin
and kaempferol, may be a useful therapy for immediate-type hypersensitivity