<p>(a) MiRNA-30c in situ hybridization on adult wildtype hearts shows expression in the nuclei of both cardiomyocytes (black arrowheads) and interstitial cells (grey arrowheads). The cytoplasm is also miRNA-30c positive. (b) Schematic overview of the miRNA-30c overexpression construct used for the generation of transgenic mice. (c) Northern blot for miRNA-30c in wildtype and transgenic littermates in line B at 8 weeks of age. U6 was used as a loading control and shows similar loading. (d) Quantification of miRNA-30c overexpression by qPCR in both transgenic lines at 4 weeks of age (N≥6). (e) MiRNA-30c expression in left en right ventricular tissue of line B at 4 weeks of age (N = 6). Error bars represent s.e.m. and * denotes a p-value ≤0.05.</p
