<p>At indicated time points RNA from draining popliteal lymph node cells of wild-type, TLR9<sup>−/−</sup> and Myd88<sup>−/−</sup> mice infected by <i>L.g.</i> LRV<sup>high</sup> (Fig3A left panel) or <i>L.g.</i> LRV<sup>low</sup> (Fig3A right panel) were reverse-transcribed into cDNA and relative transcript levels were determined using gene specific primers by quantitative real time PCR. Results are expressed as mean± SEM of the individual mice analyzed per group with the average value for the C57BL/6 mice given a value of 1. A.U refers to Arbitrary Units of fold change. (B) Mice (n≥4) were infected in the hind footpads and footpad swelling was measured weekly over 8 weeks using a Vernier caliper. (D) At 8 weeks post-infection mice were sacrificed and footpad parasite burden was determined using parasite specific Kmp11 primers. Results are expressed as the mean ± SEM. Significance determined at *p≤0.05, **p≤0.01, ***p≤0.005.</p
