<p>(A) RIPA, triton soluble (cytosolic) and triton insoluble (cytoskeletal-associated) extracts from P2 control and <i>Dlg10CRE</i> lenses were subjected to western blot analysis for Fgfr1, Fgfr2, and Fgfr3 and the blots reprobed for Gapdh as a loading control. Representative blots are shown. (B) Quantification of protein levels. Shown are the levels of each Fgfr in extracts from <i>Dlg10CRE</i> lenses relative to levels in the control (control levels set at 1.0). Signal intensities were quantified by phosphorimager analysis, as described in Materials and Methods, and the data subjected to statistical analysis using the two-sided One Sample t-test. At least 3 protein pools were analyzed in triplicate over 1–3 blots. The relative levels of Fgfr2 were reduced in the whole cell extract and cytoskeletal associated fraction compared to controls whereas the levels of Fgfr1 were increased as compared to controls. Error bars = standard deviations. * = FDR<0.05, ** = FDR<0.01.</p
