Mapping the Variation of the
Carrot Metabolome Using <sup>1</sup>H NMR Spectroscopy and Consensus
PCA
- Publication date
- Publisher
Abstract
Genetic
variation is the most influential factor for carrot (Daucus carota L.) composition. However, difference
in metabolite content between carrot varieties has not been described
by NMR, although primary metabolites are important for human health
and sensory properties. The aim of the present study was to investigate
the effect of genotype on carrot metabolite composition using a <sup>1</sup>H NMR-based metabolomics approach. After extraction using
aqueous and organic solvents, 25 hydrophilic metabolites, β-carotene,
sterols, triacylglycerols, and phospholipids were detected. Multiblock
PCA showed that three principal components could be identified for
classification of the five carrot varieties using different spectroscopic
regions and the results of the two solvent extraction methods as blocks.
The varieties were characterized by differences in carbohydrate, amino
acid, nucleotide, fatty acid, sterol, and β-carotene contents. <sup>1</sup>H NMR spectroscopy coupled with multiblock data analysis was
an efficient and useful tool to map the carrot metabolome and identify
genetic differences between varieties