<p><b>A:</b> Heatmap of upregulated UPR effector genes in nAtf6 transgenic larvae. The log values based on mRNA-Seq analysis and the median fold change in 3–6 liver samples assessed by qPCR at 5 dpf and 14 dpf. <b>B:</b> Most Atf6 targets are upregulated in the liver in response to ethanol. Genes identified as part of the UPR are significantly induced in the liver of nAtf6 TG and ethanol treated larvae (log value ≥ 0.2; see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1004335#pgen.1004335.s012" target="_blank">Table S2</a>). <b>C:</b> Oil red O stained WT and nAtf6 transgenic larvae. Livers are circled in the enlarged boxes. <b>D:</b> Steatosis incidence based on scoring of whole mount oil red O stained larvae at 4, 5, and 5.5 dpf. Statistics: chi-square with Fisher's Exact Test. *, p<0.05. “Total n” and “clutch n” corresponds to the number of larvae and number of clutches scored. <b>E:</b> Hepatic triglyceride levels in extracts from pooled livers from WT and nAtf6 transgenic larvae at 5 dpf and 14 dpf, and from single adult livers (∼9 months). Statistics: unpaired t-test. *, p<0.05. Median fold changes are noted.</p
