Figure 1

Abstract

<p>(A) Fluorescent confocal images of a single isolated FDB muscle fiber loaded with MitoSox red (125 nM) and Mitotracker green (20 nM). (i)Fluorescent image of fiber loaded with mitotracker, (ii) Fluorescent image of fiber loaded with MitoSox obtained using excitation at 405 nm, (iii) Fluorescent image of fiber loaded with MitoSox obtained using excitation at 488 nm, (iv) merge of bright field and fluorescent images, (v) merged fluorescent images i–iii, (vi) bright field image. Scale bar = 25 µm. (B) Fluorescence values from MitoSox red loaded fibers (n = 3) with time, prior to -, and after exposure to 10 µM antimycin A (denoted by the black bar). Data from excitation at 405 nm is claimed to reflect 2-hydroxy MitoSox and excitation at 488 nm monitors non-specific oxidation products of MitoSox.</p