Figure 5

Abstract

<p>(A) Cyto-<i>HyPer</i> transfected single muscle fiber, (i) Fluorescent image obtained using excitation at 405 nm, (ii) Fluorescent image obtained using excitation at 488 nm, (iii) bright field image, (iv) merged images i and ii, scale = 50 µm. (B) Non-transfected single muscle fiber, (i) Fluorescent image obtained using excitation at 405 nm, (ii) Fluorescent image obtained using excitation at 488 nm, (iii) bright field image, (iv) merged images i and ii, scale = 50 µm. (C) Ratio of fluorescence values at excitations of 488/405 nm from fibers transfected with <i>HyPer</i> that were either stimulated (as denoted by black bars) or non- stimulated (n = 7 for both groups). (D) Rate of change in ratio of fluorescence at excitations of 488/405 nm from fibers transfected with <i>HyPer</i>. *P<0.05, compared with non stimulated fibers at the same time point.</p