Figure 3

Abstract

<p>(A) DHE loaded single muscle fiber, (i) Fluorescent image obtained using excitation at 405 nm, (ii) Fluorescent image obtained using excitation at 488 nm, (iii) merged images i and ii, (iv) bright field image, scale bar = 50 µm. Figures B and D show relative fluorescence with time from skeletal muscle fiber nuclei (data presented from 405 nm excitation only, n = 8). Fibers were either at rest or subjected to muscle contraction as denoted by the black bar. Figures C and E show the rate of change in relative fluorescence (derived from figures B and D respectively) between indicated time points. Data shown in Figures B and C were from untreated fibers, data in Figures D and E were from fibers in the presence of 100 µM L-NAME. *P<0.05 compared with non-stimulated fibers at the same time point.</p