Figure 2

Abstract

<p>(A) MitoSox red loaded single isolated skeletal muscle fiber, (i) Fluorescent image obtained using excitation at 405 nm, (ii) Fluorescent image obtained using excitation at 488 nm, (iii) merged images i and ii, (iv) bright field image, scale bar = 25 µm. Figures B and D show the relative fluorescence with time from skeletal muscle fibers loaded with MitoSox red (data presented from 405 nm excitation). Fibers were either non-stimulated or subjected to two periods of electrically stimulated contractions during the time periods denoted by a black bar. No significant effect of stimulation over the whole time course was found in the absence of L-NAME (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096378#pone-0096378-g002" target="_blank">Fig. 2B</a>) whereas in the presence of L-NAME (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0096378#pone-0096378-g002" target="_blank">Fig. 2D</a>) a significant effect of stimulation was found (repeated measures, F = 5.3, P = 0.04) compared with non stimulated fibers. Figures C and E show the rate of change in relative fluorescence (derived from figures B and D ) between the indicated time points with the stimulation periods denoted by black bars. Data shown in Figures B and C were from untreated fibers, data in Figures D and E were from fibers in the presence of 100 µM L-NAME. *P<0.05 compared with non-stimulated fibers at the same time point (n = 6–7 for all groups).</p