<p>Total protein was isolated from control and PP-treated breast cancer cells and subjected to immunoblotting of proteins. Membranes were stripped and re-probed with anti-actin antibody to ensure equal protein loading. (<b>A</b>) Immunoblotting of FOXM1 after PP treatment and silencing of FOXM1 and NF-κB. (<b>B</b>) Immunoblots of cell lysates treated with PP or silenced FOXM1 and NF-kB for E cadherin, vimentin, slug and zeb-1. (<b>C</b>) Bar diagram indicate the fold difference in the EMT markers after PP treatment and above specified silencing. * indicates statistically significant difference between PP treated and untreated cells at p<0.05 (*) and p<0.01(**) levels by student's t-test. Altogether this data indicate the PP induced EMT reversal is through FOXM1 in breast cancer cells.</p
