<p>NRK cells stably expressing the fusion protein TGN38-EGFP were cultured in a flow-through chamber and observed by time-resolved fluorescence microscopy. At time point “0 min” cell were treated with DMSO (0.1%) <b>(line 1)</b>, 50 µM IC261 <b>(line 2)</b>, 5 µM nocodazole <b>(line 3)</b> or 10 µM taxol <b>(line 4)</b>. Here representative cells are shown for the stated time points (see video sequence, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0100090#pone.0100090.s005" target="_blank">movie S1</a>). The solvent DMSO and the treatment with taxol showed no effect on the TGN structure. IC261 as well as nocodazole treatment fragmented the tubular membrane structure of the TGN into vesicles distributed throughout the cell. At time point “−10 min” cells were treated with 10 µM taxol and from time point “0 min” on with 10 µM taxol +50 µM IC261 <b>(line 5)</b>. Additional treatment with taxol could prevent the IC261 induced effects on the TGN.</p
