<p>CV-1 and AC1-M88 cells were cultivated for 12, 24 and 48 h with different concentrations of IC261 (0.2–3.2 µM) or DMSO (0.0 µM) and subsequently analyzed by FACS analysis with FACScan (Becton Dickinson) (as described in Material and Methods). IC261 induced a full G2/M arrest (arrow) at a cell type dependent concentration (CV1∶1.6 µM, AC1-M88∶0.8 µM). At half of this concentration IC261 induces an increase of the subG1 population (black triangle: CV-1∶0.8 µM, AC1-M88∶0.4 µM). Interestingly, at higher concentrations the amount of cells in subG1 population is smaller indicating less apoptosis (open triangle).</p
