<p>Polysome association of mRNAs was measured by sucrose density gradient fractionation and microarray analysis of four pools (F: free, S: 40S-associated, L: light polysomes, H: heavy polysomes). For each mRNA, the distribution across the pools was calculated. The ratio H/L was used as a measure for ribosome load. mRNA levels were quantified by RNASeq at a high temporal resolution and grouped into five distinct patterns (g0–g4). By combining both data sets, mRNAs whose translation is actively regulated can be distinguished from mRNAs with passive changes in translation.</p
