<p>(A) Diagram depicting the similarity of regulatory motifs between the <i>Ier3</i> and <i>Tnf</i> genes. Shared transcriptional and post-transcriptional elements are highlighted. (B) HEK-Blue mTLR4 cells were transiently co-transfected with different Firefly Luciferase reporter genes together with a Renilla Luciferase expressing plasmid. Firefly Luciferase reporter genes contained either the rabbit β-globin (<i>HBB2</i>) 3′UTR alone, the complete <i>Ier3</i> 3′UTR or the <i>Ier3</i> 3′UTR without the ARE. (C) To determine the relative translation efficiency, Firefly Luciferase (FLuc) activity was normalized to Renilla Luciferase (RLuc) activity and to the Firefly Luciferase reporter mRNA level as determined by Northern blot analysis. Bars represent mean values ± SEM (n = 4).</p
