<p>(<b>A</b>) Subconfluent monolayers of HUVECs were infected for 48 h with MOI = 200 of the <i>Bhe</i> Δ<i>bepDEF</i> mutant complemented with the indicated Bep-expression plasmids followed by fixation, immunocytochemical staining and confocal laser scanning microscopy. F-actin is represented in red and DNA in blue (scale bar = 50 µm). (<b>B</b>) Quantification of cell fragmentation at 48 h post infection was performed as described for <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004187#ppat-1004187-g001" target="_blank">Fig. 1C and D</a> and presented as mean of triplicate samples +/− SD. Statistical significance was determined using Student's <i>t</i>-test. <i>P</i><0.05 was considered statistically significant. Data from one representative experiment (n = 3) are presented.</p
