Facile
Synthesis of Enzyme-Inorganic Hybrid Nanoflowers
and Its Application as a Colorimetric Platform for Visual Detection
of Hydrogen Peroxide and Phenol
- Publication date
- Publisher
Abstract
This
study reports a facile approach for the synthesis of horseradish
peroxidise (HRP)-inorganic hybrid nanoflowers by self-assembly of
HRP and copper phosphate (Cu<sub>3</sub>(PO<sub>4</sub>)<sub>2</sub>·3H<sub>2</sub>O) in aqueous solution. Several reaction parameters
that affect the formation of the hybrid nanoflowers were investigated
and a hierarchical flowerlike spherical structure with hundreds of
nanopetals was obtained under the optimum synthetic conditions. The
enzymatic activity of HRP embedded in hybrid naonflowers was evaluated
based on the principle of HRP catalyzing the oxidation of <i>o</i>-phenylenediamine (OPD) in the presence of hydrogen peroxide
(H<sub>2</sub>O<sub>2</sub>). The results showed that 506% enhancement
of enzymatic activity in the hybrid nanoflowers could be achieved
compared with the free HRP in solution. Taking advantages of the structural
feature with catalytic property, a nanoflower-based colorimetric platform
was newly designed and applied for fast and sensitive visual detection
of H<sub>2</sub>O<sub>2</sub> and phenol. The limits of detection
(LODs) for H<sub>2</sub>O<sub>2</sub> and phenol were as low as 0.5
μM and 1.0 μM by the naked-eye visualization, which meet
the requirements of detection of both analytes in clinical diagnosis
and environmental water. The proposed method has been successfully
applied to the analysis of low-level H<sub>2</sub>O<sub>2</sub> in
spiked human serum and phenol in sewage, respectively. The recoveries
for all the determinations were higher than 92.6%. In addition, the
hybrid nanoflowers exhibited excellent reusability and reproducibility
in cycle analysis. These primary results demonstrate that the hybrid
nanoflowers have a great potential for applications in biomedical
and environmental chemistry
