Macromolecular Sensing of RNAs by Exploiting Conformational
Changes in Supramolecular Nanostructures
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Abstract
Here,
we report on a ratiometric fluorescence biosensor based on
self-assembled peptide nanostructures (SPN), which can respond to
conformational changes induced by RNA ligand binding. The design of
the SPN biosensor was inspired by the conformational stabilization
and multimerization behaviors of the HIV-1 Rev protein induced by
cooperative protein–protein and protein–RNA interactions.
Because conformation-sensitive SPN biosensors can orchestrate binding
and signal transduction events, they can be developed as highly sophisticated
and smart nanomaterials for biosensing