New Generation of Bioorthogonally Applicable Fluorogenic
Dyes with Visible Excitations and Large Stokes Shifts
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Abstract
Synthesis of a set of new, azide
bearing, biorthogonally applicable
fluorogenic dyes with large Stokes shifts is presented herein. To
assess the fluorogenic performance of these new dyes we have labeled
a genetically modulated, cyclooctyne-bearing protein in lysate medium.
Studies showed that the labels produce specific signal with minimal
background fluorescence. We also provide theoretical insights into
the design of such fluorogenic labels