<p>The main technical limitation of the real time NIRS in vivo measurement is the difficulty in obtaining a calibrated scale for quantification purposes, because a zero setting is impossible to reach under physiological conditions. A lower arm exercise during the occlusion allows the lowest O2-Hb level to be reached as quickly as possible without causing<br>excessive discomfort due to the pressure exertion. Once the plateau is evident the exerted pressure is relieved. Although this is a rough estimate of the absolute concentration levels of O Hb and HHb, it still gives us a more accurate description of the dynamic changes of the oxygen supply at tissular level. The ischemic arrest is not a new method to evaluate the lowest O2-Hb concentration, but in order to map the oxygenation profile in a detailed way; we propose some alterations to this technique.</p>
<p> </p
