Proteome Analysis of the Inner
Integument from Developing <i>Jatropha curcas</i> L. Seeds
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Abstract
In
this study, we performed a systematic proteomic analysis of
the inner integument from developing seeds of <i>Jatropha curcas</i> and further explored the protein machinery responsible for generating
the carbon and nitrogen sources to feed the growing embryo and endosperm.
The inner integument of developing seeds was dissected into two sections
called distal and proximal, and proteins were extracted from these
sections and from the whole integument and analyzed using an EASY-nanoLC
system coupled to an ESI-LTQ-Orbitrap Velos mass spectrometer. We
identified 1526, 1192, and 1062 proteins from the proximal, distal,
and whole inner integuments, respectively. The identifications include
those of peptidases and other hydrolytic enzymes that play a key role
in developmental programmed cell death and proteins associated with
the cell-wall architecture and modification. Because many of these
proteins are differentially expressed within the integument cell layers,
these findings suggest that the cells mobilize an array of hydrolases
to produce carbon and nitrogen sources from proteins, carbohydrates,
and lipids available within the cells. Not least, the identification
of several classes of seed storage proteins in the inner integument
provides additional evidence of the role of the seed coat as a transient
source of reserves for the growing embryo and endosperm