Cobaloxime-Based Artificial Hydrogenases

Abstract

Cobaloximes are popular H₂ evolution molecular catalysts but have so far mainly been studied in nonaqueous conditions. We show here that they are also valuable for the design of artificial hydrogenases for application in neutral aqueous solutions and report on the preparation of two well-defined biohybrid species via the binding of two cobaloxime moieties, {Co­(dmgH)₂} and {Co­(dmgBF₂)₂} (dmgH₂ = dimethylglyoxime), to apo <i>Sperm-whale</i> myoglobin (<i>Sw</i>Mb). All spectroscopic data confirm that the cobaloxime moieties are inserted within the binding pocket of the <i>Sw</i>Mb protein and are coordinated to a histidine residue in the axial position of the cobalt complex, resulting in thermodynamically stable complexes. Quantum chemical/molecular mechanical docking calculations indicated a coordination preference for His93 over the other histidine residue (His64) present in the vicinity. Interestingly, the redox activity of the cobalt centers is retained in both biohybrids, which provides them with the catalytic activity for H₂ evolution in near-neutral aqueous conditions