<p>The dataset consists of the following: Force-distance curves collected during AFM application using Asylum MFP3D software in IgorPro 6.22 platform, labeled as “Force curves”<br>Video files of changes in fluorescence intensity of fluo-4 loaded cells during the indentation experiment labeled as “movie”<br>Examples of traces of changes in fluorescence intensity extracted from the video files using a code for Matlab 2011 A available in reference 22 in the associated article, labeled as “Mean Ca Fluorescence intensity”</p