Metal Mesh
Device Sensor Immobilized with a Trimethoxysilane-Containing
Glycopolymer for Label-Free Detection of Proteins and Bacteria
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Abstract
Biosensors for the detection of proteins
and bacteria have been
developed using glycopolymer-immobilized metal mesh devices. The trimethoxysilane-containing
glycopolymer was immobilized onto a metal mesh device using the silane
coupling reaction. The surface shape and transmittance properties
of the original metal mesh device were maintained following the immobilization
of the glycopolymer. The mannose-binding protein (concanavalin A)
could be detected at concentrations in the range of 10<sup>–9</sup> to 10<sup>–6</sup> mol L<sup>–1</sup> using the glycopolymer-immobilized
metal mesh device sensor, whereas another protein (bovine serum albumin)
was not detected. A detection limit of 1 ng mm<sup>–2</sup> was achieved for the amount of adsorbed concanavalin A. The glycopolymer-immobilized
metal mesh device sensor could also detect bacteria as well as protein.
The mannose-binding strain of <i>Escherichia coli</i> was
specifically detected by the glycopolymer-immobilized metal mesh device
sensor. The glycopolymer-immobilized metal mesh device could therefore
be used as a label-free biosensor showing high levels of selectivity
and sensitivity toward proteins and bacteria