<p>(A–B) Primary B cells from wild type C57BL6 (B6) mice were transduced with retroviral vectors encoding either Y120F, Y129F, wild type M2 or M2stop. On days 2 and 3 post transduction, the cells were analyzed by phosphoflow as described in materials and methods. (A) B cells from B6 mice transduced as mentioned above were analyzed for pSTAT3 levels after gating on live cells by forward and side scatter characteristics. Representative histograms of pSTAT3 levels, one each on day 2 and day 3 are shown. (B) Mean Fluorescent Intensities (MFIs) of pSTAT3 fluorescence from three independent replicates are shown for each condition shown in A.</p
