<p>(A and B) Western blot analysis of p-Smad3 and vimentin protein expression in unobstructed and obstructed kidneys from WT and Akt2 KO mice. (C and D) Immunohistochemistry for E-cadherin and α-smooth-muscle actin (α-SMA) in unobstructed and obstructed kidneys from WT and Akt2 KO mice. Magnification: ×200. (E and F) Western blot analysis of E-cadherin and α-SMA protein expression in unobstructed and obstructed kidneys from WT and Akt2 KO mice. GAPDH was used as internal loading control. Band intensities were calculated using Scion Image software. Data are shown as mean ± SD (n = 6). <sup>#</sup><i>P</i><0.05, <sup>##</sup><i>P</i><0.01 compared to the nonobstructive kidney from WT mice; <sup>*</sup><i>P</i><0.05 compared to the obstructive kidneys from WT mice.</p
