Competitive
Assembly To Increase the Performance of the DNA/Carbon-Nanomaterial-Based
Sensing Platform
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Abstract
Increasing
the rate of target binding on the surface and enhancing the fluorescence
signal restoration efficiency are critical to the desirable biomedical
application of carbon nanomaterials, for example, single-walled carbon
nanotubes (SWNTs). We describe here a strategy to increase the target
binding rate and enhance the fluorescence signal restoration efficiency
on the DNA-functionalized SWNT surface using a short complementary
DNA (scDNA) strand. The scDNA causes up to a 2.5-fold increase in
association rate and 4-fold increase in fluorescence signal restoration
by its competitive assembly on the nanostructure’s surface
and inducing a conformational change that extends the DNA away from
the surface, making it more available to bind target nucleic acids.
The scDNA-induced enhancement of binding kinetics and fluorescence
signal restoration efficiency is a general phenomenon that occurred
with all sequences and surfaces investigated. Through this competitive
assembly strategy of scDNA, performance improvement of the carbon-nanomaterial-based
biosensing platform for both in vitro detection and live cell imaging
can be reached