Immobilizing Gold Nanoparticles in Mesoporous Silica
Covered Reduced Graphene Oxide: A Hybrid Material for Cancer Cell
Detection through Hydrogen Peroxide Sensing
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Abstract
A new
kind of two-dimensional (2-D) hybrid material (RGO-PMS@AuNPs),
fabricated by the immobilization of ultrasmall gold nanoparticles
(AuNPs, ∼3 nm) onto sandwich-like periodic mesopourous silica
(PMS) coated reduced graphene oxide (RGO), was employed for both electrocatalytic
application and cancer cell detection. The hybrid-based electrode
sensor showed attractive electrochemical performance for sensitive
and selective nonenzymatic detection of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in 0.1 M phosphate buffered saline, with wide linear
detection range (0.5 μM to 50 mM), low detection limit (60 nM),
and good sensitivity (39.2 μA mM<sup>–1</sup> cm<sup>–2</sup>), and without any interference by common interfering
agents. In addition, the sensor exhibited a high capability for glucose
sensing and H<sub>2</sub>O<sub>2</sub> detection in human urine. More
interestingly, the hybrid was found to be nontoxic, and the electrode
sensor could sensitively detect a trace amount of H<sub>2</sub>O<sub>2</sub> in a nanomolar level released from living tumor cells (HeLa
and HepG2). Because the hybrid presents significant properties for
the detection of bioactive species and certain cancerous cells by
the synergistic effect from RGO, PMS, and AuNPs, it could be able
to serve as a versatile platform for biosensing, bioanalysis, and
biomedical applications