Effect of homology arm length for Cas9-mediated knockin.

Abstract

<p><b>A.</b> GFP knockin at Oct4 C-terminus using 50 bp or 200 bp homology arms. Knockin efficiency was measured by FACS for GFP expression. <b>B–C.</b> GFP expression in GFP⁺ FACS clones by microscopy and western blotting. <b>D.</b> PCR genotyping of individual ESC clones. The PCR product (arrowhead) is diagnostic of knockin at <i>Oct4</i>.</p