Splenic mDC and pDC activation by miR-29b <i>in vivo</i>.

Abstract

<p>BALB/c mice were injected intravenously with miR-29b, miR-127, or siRNA9.1. Spleens were harvested eighteen hours after injection and CD40, CD86, and H-2Kd expression was evaluated by flow cytometry, on CD11c⁺CD11b⁺B220⁻ mDC (A) or CD11c^lowCD11b⁻B220⁺ pDC (B) subsets. Histogram plots show the results of CD40, CD86 and H-2K^d staining for one mouse out of two in one experiment representative of four independent experiments. Grey shading indicates isotypic controls. For each marker, graphs represent the relative fluorescence intensity (RFI) of individual mice in two independent experiments (n = 3 mice for miR-29b and siRNA9.1, n = 4 mice for miR-127), and are representative of two other independent experiments. *<i>P</i><0.05 (Mann-Whitney).</p