<div><p>A new C<sub>19</sub>-diterpenoid alkaloid, ducloudine F (<b>1</b>), was obtained from the roots of <i>Aconitum duclouxii</i>, along with eight known alkaloids (<b>2</b>–<b>9</b>) isolated from this species for the first time. Their structures were established on the basis of extensive spectroscopic analyses. The antimicrobial activities of these compounds were investigated.</p></div