Surface Presentation of Functional Peptides in Solution
Determines Cell Internalization Efficiency of TAT Conjugated Nanoparticles
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Abstract
Functionalizing
nanoparticles with cell-penetrating peptides is
a popular choice for cellular delivery. We investigated the effects
of TAT peptide concentration and arrangement in solution on functionalized
nanoparticles’ efficacy for membrane permeation. We found that
cell internalization correlates with the positive charge distribution
achieved <i>prior</i> to nanoparticle encountering interactions
with membrane. We identified a combination of solution based properties
required to maximize the internalization efficacy of TAT-functionalized
nanoparticles