Engineered M13 Bacteriophage Nanocarriers for Intracellular
Delivery of Exogenous Proteins to Human Prostate Cancer Cells
- Publication date
- Publisher
Abstract
The
size, well-defined structure, and relatively high folding energies
of most proteins allow them to recognize disease-relevant receptors
that present a challenge to small molecule reagents. While multiple
challenges must be overcome in order to fully exploit the use of protein
reagents in basic research and medicine, perhaps the greatest challenge
is their intracellular delivery to a particular diseased cell. Here,
we describe the genetic and enzymatic manipulation of prostate cancer
cell-penetrating M13 bacteriophage to generate nanocarriers for the
intracellular delivery of functional exogenous proteins to a human
prostate cancer cell line