Periodic
Fluorescent Silver Clusters Assembled by
Rolling Circle Amplification and Their Sensor Application
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Abstract
A simple method for preparing DNA-stabilized
Ag nanoclusters (NCs)
nanowires is presented. To fabricate the Ag NCs nanowires, we use
just two unmodified component strands and a long enzymatically produced
scaffold. These nanowires form at room temperature and have periodic
sequence units that are available for fluorescence Ag NCs assembled
which formed three-way junction (TWJ) structure. These Ag NCs nanowires
can be clearly visualized by confocal microscopy. Furthermore, due
to the high efficiency of rolling circle amplification reaction in
signal amplification, the nanowires exhibit high sensitivity for the
specific DNA detection with a wide linear range from 6 to 300 pM and
a low detection limit of 0.84 pM, which shows good performance in
the complex serum samples. Therefore, these Ag NCs nanowires might
have great potential in clinical and imaging applications in the future