<p>Representative gels for A) methylation sensitive PCR of bisulfite modified DNA for the <i>NFκB</i> promoter; B) PCR of unmodified genomic DNA; and C) methylation sensitive PCR of bisulfite modified DNA for <i>TSLC1</i> and <i>CDH1</i> promoter methylation. D) Bisulfite sequencing for <i>NFκB</i> promoter. ‘L’ lanes indicate molecular weight ladders, ‘u’ lanes indicate PCR using primers specific to unmethylated promoter sequences; ‘m’ lanes indicate PCR using primers specific to methylated promoter sequences; ‘u+’ and ‘m+’ indicate respective positive control PCRs; the H2O lane indicates a PCR control containing no DNA template; open circles indicate unmethylated CpG; dashes indicate that no data was obtained).</p
