Protein quantification in aging colonies.

Abstract

<p>Total protein was prepared from colonies of either wild-type, <i>rpoB</i> P564L mutant or Δ<i>rpoS</i> mutant, all grown for 1, 3, 5 and 7 days. Concentrations of the proteins of interest were quantified by Single Ion Monitoring mass spectrometry. Bars represent averages of two or three peptides per protein, measured in biological duplicates, each measured twice, with error bars representing standard deviation between the runs. Concentration values are in arbitrary units, normalized to total protein in the samples, to enable direct comparison between different days and different samples. The day 5 sample from the Δ<i>rpoS</i> mutant could not be analyzed. Statistical significance of differences, compared to wild-type samples of the same age, are indicated in the figure (-  =  not significant, *  = 95% confidence interval, **  = 99% conficence interval, ***  = 99.9% confidence interval). <b>A</b>. Concentration of Acs, acetyl-CoA synthase. <b>B</b>. Concentration of AceA, isocitrate lyase. <b>C</b>. Concentration of AceB, malate synthase. <b>D</b>. Concentration of AckA, acetate kinase. <b>E</b>. Concentration of Pta, phosphotransacetylase.</p