<p>PBMCs were stimulated by phytohemagglutinin or by no mitogen stimulant for 24 hours. Cells were harvested and stained with fluorochrome conjugated CD3, CD4, CD8, IFN-γ, IL-2 or TNF-α mAbs. PBMCs were gated by forward and side scatter and the CD3<sup>+</sup> T lymphocyte population was identified (A). IFN-γ, IL-2 or TNF-α expression in CD4<sup>+</sup> T cells (B) or CD8+ T cells (C) were then examined. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110276#pone-0110276-g002" target="_blank">Figures 2D and 2E</a> show fold increase of IFN-γ, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110276#pone-0110276-g002" target="_blank">Figures 2F and 2G</a> fold increases of IL-2, and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110276#pone-0110276-g002" target="_blank">Figures 2H and 2I</a> fold increases of TNF-α production in response to PHA, compared to cells cultured with no mitogen stimulant. Symbols represents the data of each individual case patient (circles), HIV+ control (up-pointing triangles), and healthy control (down-pointing triangles). Long horizontal lines show the medians, and short lines the interquartile ranges of each group. Indicated <i>p</i> values for comparisons are by Kruskal-wallis one-way analysis of variance by rank test.</p
