<p>(A) Construction of iBrick parts, which should be prefixed and suffixed in iBrick standard. (B) Forward assembly procedure. Part B is firstly released with I-SceI and PI-PspI digestion, and is then inserted into the PI-PspI digested vector, obtaining an assembled part AB. Alternatively, the vector can also be cut with I-SceI followed by insertion of part B to obtain part BA, which is designated the reverse assembly.</p
