On-Chip Determination of C‑Reactive Protein
Using Magnetic Particles in Continuous Flow
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Abstract
We demonstrate the application of
a multilaminar flow platform,
in which functionalized magnetic particles are deflected through alternating
laminar flow streams of reagents and washing solutions via an external
magnet, for the rapid detection of the inflammatory biomarker, C-reactive
protein (CRP). The two-step sandwich immunoassay was accomplished
in less than 60 s, a vast improvement on the 80–300 min time
frame required for enzyme-linked immunosorbent assays (ELISA) and
the 50 min necessary for off-chip magnetic particle-based assays.
The combination of continuous flow and a stationary magnet enables
a degree of autonomy in the system, while a detection limit of 0.87
μg mL<sup>–1</sup> makes it suitable for the determination
of CRP concentrations in clinical diagnostics. Its applicability was
further proven by assaying real human serum samples and comparing
those results to values obtained using standard ELISA tests
