Proteomic Analysis of Proteins
Related to Prognosis
of Lung Adenocarcinoma
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Abstract
We attempted to identify prognosis-related
proteins expressed in
early resection lung adenocarcinomas that had higher metastatic potential.
Early resection of lung adenocarcinoma tissues were collected from
patients who experienced recurrence within 5 years after surgery;
these patients are defined here as the poor prognosis group. From
these samples, we prepared frozen tissue sections and then isolated
cancerous areas by laser capture microdissection to allow extraction
of cancer tissue-derived soluble proteins. Shotgun LC–MS/MS
analysis detected and identified a total of 875 proteins in these
cancer tissues. Relative quantitative analysis revealed that 17 proteins
were preferentially expressed in the poor prognosis group relative
to the good prognosis group, which consisted of patients who did not
exhibit recurrence. Among them, 14-3-3 beta/alpha and calnexin were
reported to be potentially involved in tumor recurrence and the malignant
properties of lung cancer. Here immunological analyses confirmed disease-associated
expression of these proteins. In a cell-culture model using A549,
targeted depletion of either 14-3-3 beta/alpha or calnexin reduced
proliferation, invasion, and migration, suggesting that both proteins
are involved in determining the malignant properties of lung cancer
that contribute to poor prognosis