<p>The sequences of NS4B amino acids (aa) 1–70 from the HCV JFH1 strain (genotype 2a; UniProtKB entry Q99IB8) and the non primate hepacivirus strain NPHV-B10-022 (UniProtKB entry I1ZAS8) were aligned by ClustalW (see Legend to <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1004501#ppat-1004501-g001" target="_blank">Figure 1</a> for color code). The similarity index according to ClustalW convention is shown below (asterisk, invariant; colon, highly similar; dot, similar). Helical wheel projections of aa 7–33 of HCV JFH1 and NPHV-B10-022 are shown in the lower panel. The structurally conserved positively charged aa residues flanking the amphipathic α-helices (K, lysine; R, arginine) as well as the glutamate (E) residues on the hydrophilic side are highlighted.</p
