Flagellin protein secretion is restored in the absence of FliHIJ ATPase components by Δ<i>atpA</i>, <i>ΔflgM</i>, <i>ΔclpX</i>, and <i>fliA<sup>H14D</sup></i> mutations.

Abstract

<p>Secreted FliC flagellin protein was analyzed by anti-FliC immunostaining in the FliC-phase locked wildtype and <i>fliHIJ</i> mutant strains. (<b>A</b>) Wildtype and <i>fliHIJ</i> mutants in combination with Δ<i>atpA</i>. (<b>B</b>) Wildtype and <i>fliHIJ</i> mutants in combination with Δ<i>flgM</i> and Δ<i>atpA</i>. (<b>C</b>) Wildtype and <i>fliHI</i> deletion mutant in combination with Δ<i>clpX</i> and Δ<i>atpA</i>. (<b>D</b>) Wildtype and <i>fliHI</i> deletion mutant in combination with <i>fliA<sup>H14D</sup></i> and Δ<i>atpA</i>.</p

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