Abstract

<p>The morphology of the Golgi apparatus in>50 cells was analysed and quantified. We observed that, in the BiFC assay, E35K and E57K mutants displayed increased Golgi fragmentation (<b>A</b>). In the aggregation model, Golgi morphology appeared normal, displaying a compact appearance near the nucleus <b>(B). Levels of BiP in the oligomerization assay (C) and in the aggregation model (E), assessed by immunoblot analysis and respective quantifications (D and E).</b> n = 3. Student's <i>t</i> test (*p<0.05, **p<0.01, ***p<0.001).</p

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