<p><b>A. Constructs used in the aggregation model.</b> This model consists of co-expressing SynT together with synphilin-1. <b>B. Inclusion pattern in H4 cells.</b> Different SynT mutants resulted in the formation of distinct inclusion formation in human H4 cells. Scale bar: 10 µm. <b>C. Inclusion quantification.</b>>50 cells were scored per experiment and classified in different groups according to the pattern of inclusions. Representative cells were drawn to show type of inclusions present in each categories. Lysine mutants (E35K, E57K) increase the percentage of cells with inclusions and the number of inclusions per cell, whereas A30P and proline mutants reduce percentage of cells with inclusions and also the number of inclusions per cell. <b>D-E. Levels of ASYN.</b> Immunoblot analysis of the expression levels of ASYN. Student's <i>t</i> test (*p<0.05, **p<0.01, ***p<0.001). n = 3.</p
