<p>(A) Schema of the experimental design. After confirmation of tumor growth, the PDOXs were randomized to 4 groups: BLS only; BLS+NAC; FGS only; or FGS+NAC. Each treatment arm involved 8 tumor-bearing mice. The mice randomized to the NAC groups were treated with GEM (80 mg/kg) on day 8, 15 and 22. All animals underwent surgery on day 29. BLS was performed under standard bright-field using the MVX10 microscope. Fifty µg of anti-CA19-9 antibody conjugated with DyLight 650 was injected in the tail vain of mice with tumors in the FGS group 24 hours before surgery. FGS was performed using the MINI MAGLITE LED PRO flash light (MAG INSTRUMENT, Ontario, CA, USA) with excitation filter ET640/30X (Chroma Technology Corporation, Bellows Falls, VT, USA) and a Canon EOS 60D digital camera with an EF–S18–55 IS lens (Canon, Tokyo, Japan) and emission filter HQ700/75M-HCAR (Chroma Technology Corporation) under fluorescence navigation (B). Twelve weeks after surgery, animals underwent laparotomy, and the tumors were imaged, weighed and harvested for analysis. Scale bars: 2 cm (filters) and 5 cm (flash light).</p
